2P107 DNA Measurements by Using Fluorescence Correlation Spectroscopy and Two-Color Fluorescence Cross Correlation Spectroscopy

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

This article focuses on methods based on fluctuation correlation spectroscopy to determine the formation of protein complexes in living cells. We present the principles of the fluctuation method applied to cells. We discuss the novelty and the promises of this approach. The emphasis is in the discussion of the underlying statistical assumptions of the image correlation spectroscopy analysis rat...

Dual-color total internal reflection fluorescence cross-correlation spectroscopy.

We present the development and first application of a novel dual-color total internal reflection (TIR) fluorescence system for single-molecule coincidence analysis and fluorescence cross-correlation spectroscopy (FCCS). As a performance analysis, we measured a synthetic DNA-binding assay, demonstrating this dual-color TIR-FCCS approach to be a suitable method for measuring coincidence assays su...

Fluorescence correlation spectroscopy.

Two-photon standing-wave fluorescence correlation spectroscopy.

A fluorescence correlation spectroscopy experiment that combines two-photon excitation and a standing-wave interference pattern is presented. The experimental correlation function can be analyzed using a simple expression involving (1) an exponential decay with time constant tau(f), which reflects diffusion across the interference fringes, and (2) a longer-lived decay with time constant tau(ome...

Multiphoton Fluorescence Correlation Spectroscopy (MPFCS)

Nanoparticles have been demonstrated as a promising new generation of specific imaging agents and targeted delivery vehicles for the diagnosis and treatment of solid tumors. Nonetheless, for optimal therapy of tumors, the nanoparticles must penetrate the tumor interstitial matrix to reach the cancer cells. This thesis describes the development of a multiphoton fluorescence correlation spectrosc...